ARG59465

anti-SMURF1 antibody

anti-SMURF1 antibody for Western blot,Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes SMURF1
Tested Reactivity Hu, Rat
Predict Reactivity Hm
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SMURF1
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 326-351 of Human SMURF1. (DHNNRTTQFTDPRLHHIMNHQCQLKE)
Conjugation Un-conjugated
Alternate Names hSMURF1; SMAD ubiquitination regulatory factor 1; SMAD-specific E3 ubiquitin-protein ligase 1; EC 6.3.2.-; E3 ubiquitin-protein ligase SMURF1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:150 - 1:500
ICC/IF1:200 - 1:1000
IHC-P1:200 - 1:1000
WB0.1 - 0.5 µg/ml
Application Note IHC-P: Antigen Retrieval: Heat mediation was performed in EDTA buffer (pH 8.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 5% BSA.
Preservative 0.05% Sodium azide
Stabilizer 5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 57154 Human SMURF1

Swiss-port # Q9HCE7 Human E3 ubiquitin-protein ligase SMURF1

Gene Symbol SMURF1
Gene Full Name SMAD specific E3 ubiquitin protein ligase 1
Background This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Dec 2010]
Function E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. [UniProt]
Cellular Localization Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. [UniProt]
Calculated MW 86 kDa
PTM Auto-ubiquitinated in presence of NDFIP1 (PubMed:23087404). Ubiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site. [UniProt]