ARG59795

anti-SNAP91 / AP180 antibody

anti-SNAP91 / AP180 antibody for Western blot and Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes SNAP91 / AP180
Tested Reactivity Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SNAP91 / AP180
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 321-600 of Human SNAP91 (NP_001229723.1).
Conjugation Un-conjugated
Alternate Names 91 kDa synaptosomal-associated protein; Phosphoprotein F1-20; CALM; Clathrin coat assembly protein AP180; Clathrin coat-associated protein AP180; AP180

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Rat brain and Mouse brain
Observed Size 100 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20616 Mouse SNAP91

GeneID: 65178 Rat SNAP91

Swiss-port # Q05140 Rat Clathrin coat assembly protein AP180

Swiss-port # Q61548 Mouse Clathrin coat assembly protein AP180

Gene Symbol SNAP91
Gene Full Name synaptosomal-associated protein, 91kDa
Function Adaptins are components of the adapter complexes which link clathrin to receptors in coated vesicles. Clathrin-associated protein complexes are believed to interact with the cytoplasmic tails of membrane proteins, leading to their selection and concentration. Binding of AP180 to clathrin triskelia induces their assembly into 60-70 nm coats (By similarity). [UniProt]
Cellular Localization Cell membrane. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Note=Component of the coat surrounding the cytoplasmic face of coated vesicles in the plasma membrane. [UniProt]
Calculated MW 93 kDa
PTM Thr-310 can be modified by the addition of N-acetylglucosamine which can be further phosphorylated. There is no evidence for direct Thr-310 phosphorylation (By similarity). [UniProt]