ARG53604

anti-STAT3 antibody

anti-STAT3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody

Overview

Product Description

Rabbit Polyclonal antibody recognizes STAT3

Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name STAT3
Antigen Species Human
Immunogen Synthetic pepteide (19 aa) within aa. 170 - 220 of Human STAT3.
Conjugation Un-conjugated
Alternate Names ADMIO; APRF; HIES; Acute-phase response factor; Signal transducer and activator of transcription 3

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100
IPAssay-Dependent
WB1 - 2 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human Small Intestine Tissue Lysate

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20848 Mouse STAT3

GeneID: 25125 Rat STAT3

GeneID: 6774 Human STAT3

Gene Symbol STAT3
Gene Full Name signal transducer and activator of transcription 3 (acute-phase response factor) 
Background STATs (signal transducers and activators of transcription) are a family of cytoplasmic latent transcription factors that are activated to regulate gene expression in response to a large number of extracellular signaling polypeptides including cytokines, interferons, and growth factors. After phosphorylation by JAK tyrosine kinases, STATs enter the nucleus to regulate transcription of many different genes. Among the seven STATs (STAT1, STAT2, STAT3, STAT4, STAT5a, STAT5b, and STAT6), STAT1, STAT3, STAT5a, and STAT5b have a wide activation profile. STAT3 signals are pivotal to the proliferation and differentiation of neural stem cells and also participate in neuronal regeneration and cancers of the nervous system.
Function Signal transducer and transcription activator that mediates cellular responses to interleukins, KITLG/SCF and other growth factors. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4. Binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA. Cytoplasmic STAT3 represses macroautophagy by inhibiting EIF2AK2/PKR activity. Plays an important role in host defense in methicillin-resistant S.aureus lung infection by regulating the expression of the antimicrobial lectin REG3G (By similarity). [UniProt]
Cellular Localization Cytoplasm, Nucleus
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
Calculated MW 88 kDa
PTM Tyrosine phosphorylated upon stimulation with EGF. Tyrosine phosphorylated in response to constitutively activated FGFR1, FGFR2, FGFR3 and FGFR4 (By similarity). Activated through tyrosine phosphorylation by BMX. Tyrosine phosphorylated in response to IL6, IL11, LIF, CNTF, KITLG/SCF, CSF1, EGF, PDGF, IFN-alpha, LEP and OSM. Activated KIT promotes phosphorylation on tyrosine residues and subsequent translocation to the nucleus. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity. ARL2BP may participate in keeping the phosphorylated state of STAT3 within the nucleus. Upon LPS challenge, phosphorylated within the nucleus by IRAK1. Upon erythropoietin treatment, phosphorylated on Ser-727 by RPS6KA5. Phosphorylation at Tyr-705 by PTK6 or FER leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates IL6/interleukin-6 signaling.
Acetylated on lysine residues by CREBBP. Deacetylation by LOXL3 leads to disrupt STAT3 dimerization and inhibit STAT3 transcription activity (PubMed:28065600). Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated (PubMed:28065600).
Some lysine residues are oxidized to allysine by LOXL3, leading to disrupt STAT3 dimerization and inhibit STAT3 transcription activity (PubMed:28065600). Oxidation of lysine residues to allysine on STAT3 preferentially takes place on lysine residues that are acetylated (PubMed:28065600).