ARG59257
anti-SUB1 / PC4 antibody
anti-SUB1 / PC4 antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes SUB1 / PC4 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | SUB1 / PC4 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-127 of Human SUB1 / PC4 (NP_006704.3). |
| Conjugation | Un-conjugated |
| Alternate Names | Positive cofactor 4; Activated RNA polymerase II transcriptional coactivator p15; PC4; p14; P15; SUB1 homolog |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | HL60 | ||||||||
| Observed Size | 16 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P11031 Mouse Activated RNA polymerase II transcriptional coactivator p15 Swiss-port # P53999 Human Activated RNA polymerase II transcriptional coactivator p15 |
|---|---|
| Gene Symbol | SUB1 |
| Gene Full Name | SUB1 homolog, transcriptional regulator |
| Function | General coactivator that functions cooperatively with TAFs and mediates functional interactions between upstream activators and the general transcriptional machinery. May be involved in stabilizing the multiprotein transcription complex. Binds single-stranded DNA. Also binds, in vitro, non-specifically to double-stranded DNA (ds DNA). [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 14 kDa |
| PTM | Activity is controlled by protein kinases that target the regulatory region. Phosphorylation inactivates both ds DNA-binding and cofactor function, but does not affect binding to ssDNA. Seems to be phosphorylated in vivo by CK2 in at least 7 sites in the N-terminal Ser-rich region. [UniProt] |
