ARG41871

anti-SUZ12 antibody

anti-SUZ12 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Immunoprecipitation and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes SUZ12
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name SUZ12
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 470-739 of Human SUZ12. (NP_056170.2)
Conjugation Un-conjugated
Alternate Names Polycomb protein SUZ12; Chromatin precipitated E2F target 9 protein; ChET 9 protein; CHET9; Joined to JAZF1 protein; JJAZ1; Suppressor of zeste 12 protein homolog

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:100
IHC-P1:50 - 1:200
IP1:50 - 1:100
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa
Observed Size ~ 100 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 23512 Human SUZ12

GeneID: 52615 Mouse SUZ12

Swiss-port # Q15022 Human Polycomb protein SUZ12

Swiss-port # Q80U70 Mouse Polycomb protein Suz12

Gene Symbol SUZ12
Gene Full Name SUZ12 polycomb repressive complex 2 subunit
Background This zinc finger gene has been identified at the breakpoints of a recurrent chromosomal translocation reported in endometrial stromal sarcoma. Recombination of these breakpoints results in the fusion of this gene and JAZF1. The protein encoded by this gene contains a zinc finger domain in the C terminus of the coding region. [provided by RefSeq, Jul 2009]
Function Polycomb group (PcG) protein. Component of the PRC2/EED-EZH2 complex, which methylates 'Lys-9' (H3K9me) and 'Lys-27' (H3K27me) of histone H3, leading to transcriptional repression of the affected target gene. The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems. Genes repressed by the PRC2/EED-EZH2 complex include HOXC8, HOXA9, MYT1 and CDKN2A. [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 83 kDa
PTM Sumoylated, probably by PIAS2. [UniProt]