ARG63722

anti-Semaphorin 3E antibody

anti-Semaphorin 3E antibody for IHC-Formalin-fixed paraffin-embedded sections and Human

Neuroscience antibody

Overview

Product Description Goat Polyclonal antibody recognizes Semaphorin 3E
Tested Reactivity Hu
Tested Application IHC-P
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Semaphorin 3E
Antigen Species Human
Immunogen C-KPEHYRLPRHTLDS
Conjugation Un-conjugated
Alternate Names Semaphorin-3E; SEMAH; M-SemaK; coll-5; M-SEMAH

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P2 - 4 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 9723 Human SEMA3E

Swiss-port # O15041 Human Semaphorin-3E

Background Semaphorins are a large family of conserved secreted and membrane associated proteins which possess a semaphorin (Sema) domain and a PSI domain (found in plexins, semaphorins and integrins) in the N-terminal extracellular portion. Based on sequence and structural similarities, semaphorins are put into eight classes: invertebrates contain classes 1 and 2, viruses have class V, and vertebrates contain classes 3-7. Semaphorins serve as axon guidance ligands via multimeric receptor complexes, some (if not all) containing plexin proteins. This gene encodes a class 4 semaphorin. This gene encodes a class 3 semaphorin. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010]
Research Area Neuroscience antibody
Calculated MW 89 kDa