ARG23518
anti-Semaphorin 4D antibody [A8] (APC)
anti-Semaphorin 4D antibody [A8] (APC) for Flow cytometry and Human,Rhesus Monkey
Overview
| Product Description | APC-conjugated Mouse Monoclonal antibody [A8] recognizes Semaphorin 4D. Mouse anti Human CD100 antibody, clone A8 recognizes human Sepaphorin 4D, also known as CD100, A8, BB18 or GR3. CD100 is a 841 amino acid ~150 kDa homodimeric single pass type 1 transmembrane cell surface glycoprotein actin as a receptor for some members of the plexin family. CD100 is expressed at low levels by peripheral blood T cells, and at higher density on activated T cells and B cells, it is also expresses at high levels in fetal kidney with lower levels in fetal brain, liver and lung (Maestrini et al. 1996). |
|---|---|
| Tested Reactivity | Hu, R. Mk |
| Tested Application | FACS |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | A8 |
| Isotype | IgG1 |
| Target Name | Semaphorin 4D |
| Antigen Species | Human |
| Immunogen | Human PHA activated lymphocytes. |
| Conjugation | APC |
| Alternate Names | CD100; GR3; CD antigen CD100; M-sema-G; BB18; C9orf164; SEMAJ; Semaphorin-4D; A8; coll-4 |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS, 0.09% Sodium azide, 1% BSA and 5% Sucrose. |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 1% BSA and 5% Sucrose |
| Concentration | 0.1 mg/ml |
| Storage Instruction | Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SEMA4D |
| Gene Full Name | sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D |
| Function | Cell surface receptor for PLXN1B and PLXNB2 that plays an important role in cell-cell signaling. Promotes reorganization of the actin cytoskeleton and plays a role in axonal growth cone guidance in the developing central nervous system. Regulates dendrite and axon branching and morphogenesis. Promotes the migration of cerebellar granule cells and of endothelial cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Promotes signaling via SRC and PTK2B/PYK2, which then mediates activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Interaction with PLXNB1 mediates activation of RHOA. [UniProt] |
| Calculated MW | 96 kDa |
