ARG52422
anti-Serotonin Transporter phospho (Thr 276) antibody
anti-Serotonin Transporter phospho (Thr 276) antibody for Western blot and Rat
Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Serotonin Transporter phospho (Thr 276) |
|---|---|
| Tested Reactivity | Rat |
| Predict Reactivity | Hu, Ms, Bov, NHuPrm, Sheep, Xenopus laevis |
| Tested Application | WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Serotonin Transporter |
| Antigen Species | Rat |
| Immunogen | Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr276 conjugated to KLH |
| Conjugation | Un-conjugated |
| Alternate Names | SERT; 5HTT; SERT1; Sodium-dependent serotonin transporter; hSERT; 5-HTTLPR; Solute carrier family 6 member 4; HTT; 5-HTT; 5HT transporter; OCD1 |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~76k SERT protein phosphorylated at Ser 276. Immunolabeling is blocked by preadsorption of antibody with the phospho-peptide that was used to geneRate the antibody but not by the corresponding dephospho-peptide. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P31652 Rat Sodium-dependent serotonin transporter |
|---|---|
| Gene Symbol | Slc6a4 |
| Gene Full Name | solute carrier family 6 (neurotransmitter transporter), member 4 |
| Background | The serotonin transporter (SERT) recycles serotonin by transporting it back to the pre-synaptic cell. It is the primary target for most anti-depressant drugs and for stimulants such as methamphetamines. SERT is regulated by several processes, including a cyclic GMP signaling pathway involving nitric oxide synthase, guanylyl cyclase, and cGMP-dependent protein kinase (PKG). cGMP- and PKG-mediated SERT regulation requires phosphorylation at thr276 (Ramamoorthy et al., 2007). It has been suggested that although PKG is involved in the stimulation of SERT at thr276, it does not directly phosphorylate the residue, rather it initiates a kinase cascade that leads to SERT phosphorylation by an as yet unidentified protein kinase (Wong et al., 2012). Also of therapeutic importance, mutation at the thr276 residue has been shown to decrease the potency of a variety of anti-depressant drugs, (Zhang YW and Rudnick G, 2005). |
| Research Area | Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 70 kDa |
| PTM | Glycosylated; modification with sialylated N-glycans is a requirement for transporters to associate with each other and to function as homooligomeric forms. Phosphorylation at Thr-276 increases 5-HT uptake and is required for cGMP-mediated SERT regulation. Phosphorylation upon PKC stimulation modifies the SERT distribution and density in the membrane, and diminishes the uptake capacity. |
