ARG53971
anti-Sialyl Tn antibody [STn 219]
anti-Sialyl Tn antibody [STn 219] for Flow cytometry,ICC/IF,Immunohistochemistry and Human
Cancer antibody; Controls and Markers antibody; sTn Antigen antibody; Tn Antigen antibody
Publication1
Overview
| Product Description | Mouse Monoclonal antibody [STn 219] recognizes Sialyl Tn |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC |
| Specificity | This antibody stains colon carcinomas and ovarian mucinous carcinomas producing apical cytoplasmic, apical membrane or luminal staining patterns. It is negative with majority of adult tissue sections. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | STn 219 |
| Isotype | IgG1, kappa |
| Target Name | Sialyl Tn |
| Immunogen | Ovine submaxillary mucin (OSM) |
| Epitope | NeuAc α GalNacOSer/Thr |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | IHC, ICC (frozen or formalin–fixed paraffin-embedded (FFPE) tissue sections, cell smears. IHC: Use streptavidin~biotin system or polymer system, incubate 30 minutes at room temperature (1:50 - 1:100). IF, FITC~conjugated antibodies 10-20 µg/ml (1:10 - 1:20), incubatein the dark for 2 hours at RT or overnight at 4°C. Flow: FITC~conjugated antibodies, 0.2-1.0 µg/0.1 ml (1:200 - 1:1000). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Positive Control | Human gastrointestinal tumor, prostate, ovary ca |
Properties
| Form | Liquid |
|---|---|
| Buffer | PBS (pH 7.4), 1% BSA and 0.05% Sodium azide |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 1% BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | Sialsyl Tn antigen also known as CD175s is expressed in the majority of colon, gastric, breast and lung carcinoma. The sialsyl Tn antigen has a limited expression to being absent in normal colon, pancreas, stomach, ovary, and prostate. This antigen is detected in several adenocarcinomas. It appears to be useful marker for poorly differentiated colon adenocarcinomas. |
|---|---|
| Cellular Localization | Cytoplasmic, apical membrane, luminal |
| Highlight | Related Antibody Duos and Panels: ARG30015 Tn Antigen Antibody Duo (sTn, Tn) Related products: Sialyl Tn antibodies; Sialyl Tn Duos / Panels; Anti-Mouse IgG secondary antibodies; |
| Research Area | Cancer antibody; Controls and Markers antibody; sTn Antigen antibody; Tn Antigen antibody |
| Calculated MW | several HM weight glycoproteins |
Specific References
Shigella flexneri Targets Human Colonic Goblet Cells by O Antigen Binding to Sialyl-Tn and Tn Antigens via Glycan-Glycan Interactions.
ICC/IF / Human
