ARG23301

anti-Siglec 10 antibody [5G6]

anti-Siglec 10 antibody [5G6] for Flow cytometry and Human

Overview

Product Description Mouse Monoclonal antibody [5G6] recognizes Siglec 10
Tested Reactivity Hu
Tested Application FACS
Host Mouse
Clonality Monoclonal
Clone 5G6
Isotype IgG1
Target Name Siglec 10
Antigen Species Human
Immunogen Recombinant Human Siglec-10, fused with the Fc region of Human IgG.
Conjugation Un-conjugated
Alternate Names PRO940; Siglec-10; SIGLEC-10; Siglec-like protein 2; SLG2; Sialic acid-binding Ig-like lectin 10

Application Instructions

Application Suggestion
Tested Application Dilution
FACSNeat
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS and 0.09% Sodium azide.
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 89790 Human SIGLEC10

Swiss-port # Q96LC7 Human Sialic acid-binding Ig-like lectin 10

Gene Symbol SIGLEC10
Gene Full Name sialic acid binding Ig-like lectin 10
Background SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6 (MIM 604405).[supplied by OMIM, Jul 2002]
Function Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. [UniProt]
Calculated MW 77 kDa
PTM Phosphorylation of Tyr-667 is involved in binding to PTPN6. [UniProt]