ARG23301
anti-Siglec 10 antibody [5G6]
anti-Siglec 10 antibody [5G6] for Flow cytometry and Human
Overview
| Product Description | Mouse Monoclonal antibody [5G6] recognizes Siglec 10 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5G6 |
| Isotype | IgG1 |
| Target Name | Siglec 10 |
| Antigen Species | Human |
| Immunogen | Recombinant Human Siglec-10, fused with the Fc region of Human IgG. |
| Conjugation | Un-conjugated |
| Alternate Names | PRO940; Siglec-10; SIGLEC-10; Siglec-like protein 2; SLG2; Sialic acid-binding Ig-like lectin 10 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q96LC7 Human Sialic acid-binding Ig-like lectin 10 |
|---|---|
| Gene Symbol | SIGLEC10 |
| Gene Full Name | sialic acid binding Ig-like lectin 10 |
| Background | SIGLECs are members of the immunoglobulin superfamily that are expressed on the cell surface. Most SIGLECs have 1 or more cytoplasmic immune receptor tyrosine-based inhibitory motifs, or ITIMs. SIGLECs are typically expressed on cells of the innate immune system, with the exception of the B-cell expressed SIGLEC6 (MIM 604405).[supplied by OMIM, Jul 2002] |
| Function | Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, may act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules. [UniProt] |
| Calculated MW | 77 kDa |
| PTM | Phosphorylation of Tyr-667 is involved in binding to PTPN6. [UniProt] |
