ARG64758

anti-Siglec 8 antibody

anti-Siglec 8 antibody for Western blot and Human

Immune System antibody; Signaling Transduction antibody

Overview

Product Description Goat Polyclonal antibody recognizes Siglec 8
Tested Reactivity Hu
Tested Application WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name Siglec 8
Antigen Species Human
Immunogen C-SYKSQLNYKTKQ
Conjugation Un-conjugated
Alternate Names Siglec-8; Sialic acid-binding Ig-like lectin 8; SIGLEC-8; SAF-2; Sialoadhesin family member 2; SAF2; SIGLEC8L

Application Instructions

Application Suggestion
Tested Application Dilution
WB0.3 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 27181 Human SIGLEC8

Swiss-port # Q9NYZ4 Human Sialic acid-binding Ig-like lectin 8

Background Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM, May 2010]
Research Area Immune System antibody; Signaling Transduction antibody
Calculated MW 54 kDa