ARG22574
anti-Skeletal Troponin T antibody [T1/61]
anti-Skeletal Troponin T antibody [T1/61] for IHC-Frozen sections,ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Chicken
Overview
| Product Description | Mouse Monoclonal antibody [T1/61] recognizes Skeletal Troponin T This antibody recognizes the canonical isoform 1, binding to othe potential isoforms has not been evaluated (UniProt : P12620). |
|---|---|
| Tested Reactivity | Hu, Chk |
| Species Does Not React With | Rb |
| Tested Application | ELISA, ICC/IF, IHC-Fr, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | T1/61 |
| Isotype | IgG1 |
| Target Name | Skeletal Troponin T |
| Antigen Species | Chicken |
| Immunogen | Troponin T, purified from chicken breast muscle |
| Conjugation | Un-conjugated |
| Alternate Names | fTnT; Fast skeletal muscle troponin T; Troponin T, fast skeletal muscle; TnTf; TNTF; Beta-TnTF |
Application Instructions
| Application Suggestion |
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| Application Note | IHC: This product does not require protein digestion pre-treatment of paraffin sections.This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | TNNT3 |
| Gene Full Name | troponin T3, fast skeletal type |
| Background | The binding of Ca(2+) to the trimeric troponin complex initiates the process of muscle contraction. Increased Ca(2+) concentrations produce a conformational change in the troponin complex that is transmitted to tropomyosin dimers situated along actin filaments. The altered conformation permits increased interaction between a myosin head and an actin filament which, ultimately, produces a muscle contraction. The troponin complex has protein subunits C, I, and T. Subunit C binds Ca(2+) and subunit I binds to actin and inhibits actin-myosin interaction. Subunit T binds the troponin complex to the tropomyosin complex and is also required for Ca(2+)-mediated activation of actomyosin ATPase activity. There are 3 different troponin T genes that encode tissue-specific isoforms of subunit T for fast skeletal-, slow skeletal-, and cardiac-muscle. This gene encodes fast skeletal troponin T protein; also known as troponin T type 3. Alternative splicing results in multiple transcript variants encoding additional distinct troponin T type 3 isoforms. A developmentally regulated switch between fetal/neonatal and adult troponin T type 3 isoforms occurs. Additional splice variants have been described but their biological validity has not been established. Mutations in this gene may cause distal arthrogryposis multiplex congenita type 2B (DA2B). [provided by RefSeq, Oct 2009] |
| Function | Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [UniProt] |
| Calculated MW | 32 kDa |
