ARG45050

anti-Smad 1 phospho (Ser463 / 465)+ Smad 5 phospho (Ser463 / 465) + Smad 9 phospho (Ser465 / 467) antibody [RM487]

anti-Smad 1 phospho (Ser463 / 465)+ Smad 5 phospho (Ser463 / 465) + Smad 9 phospho (Ser465 / 467) antibody [RM487] for Western blot and Human

Overview

Product Description Rabbit monoclonal [RM487] recognizes Smad 1 + 5 + 9.
Tested Reactivity Hu
Tested Application WB
Specificity This antibody reacts to Smad1 and Smad5 when phosphorylated at Ser463/465 and Smad9 (Smad8) when phosphorylated at Ser465/467. There is no cross-reactivity to Smad1, Smad5, or Smad9 that are not phosphorylated. This antibody reacts to human, mouse or rat Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467).
Host Rabbit
Clonality Monoclonal
Clone RM487
Isotype IgG
Target Name Smad 1 + 5 + 9
Immunogen A phospho-peptide corresponding to human Phospho-Smad1 (Ser463/465)/ Smad5 (Ser463/465)/ Smad9 (Ser465/467)
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS with 50% Glycerol, 1% BSA and 0.09% sodium azide
Preservative 0.09% sodium azide
Stabilizer 50% Glycerol, 1% BSA and 0.09%
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.