ARG54040

anti-Smad 5 antibody (C-term)

anti-Smad 5 antibody (C-term) for Flow cytometry,ICC/IF,Western blot and Human

Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
nuts_pic
3

Overview

Product Description Mouse Monoclonal antibody recognizes Smad 5
Tested Reactivity Hu
Tested Application FACS, ICC/IF, WB
Host Mouse
Clonality Monoclonal
Isotype IgG1
Target Name Smad 5
Antigen Species Human
Immunogen Purified recombinant human SMAD5 (C-terminus) protein fragments expressed in E.coli.
Conjugation Un-conjugated
Alternate Names DWFC; Smad5; JV5-1; Mothers against decapentaplegic homolog 5; Mothers against DPP homolog 5; hSmad5; MADH5; SMAD 5; MAD homolog 5; SMAD family member 5

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:100
ICC/IF1:75
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified
Buffer 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol
Preservative 0.2% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 4090 Human SMAD5

Swiss-port # Q99717 Human Mothers against decapentaplegic homolog 5

Gene Symbol SMAD5
Gene Full Name SMAD family member 5
Background Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD).
Function Transcriptional modulator activated by BMP (bone morphogenetic proteins) type 1 receptor kinase. SMAD5 is a receptor-regulated SMAD (R-SMAD). [UniProt]
Cellular Localization Cytoplasm. Nucleus
Research Area Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
Calculated MW 52 kDa
PTM Phosphorylated on serine by BMP (bone morphogenetic proteins) type 1 receptor kinase.
Ubiquitin-mediated proteolysis by SMAD-specific E3 ubiquitin ligase SMURF1.

Customer's Feedback

nuts_pic      Excellent

Review for anti-Smad 5 antibody (C-term)

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:75

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:FITC

nuts_pic      Excellent

Review for anti-Smad 5 antibody (C-term)

Application:IF/ICC

Sample:HeLa

Fixation Buffer:100% Methanol

Fixation Time:10 min

Fixation Temperature:RT ºC

Permeabilization Buffer:0.1% Triton X-100

Primary Antibody Dilution Factor:1:75

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Conjugation of Secondary Antibody:FITC

nuts_pic      Good

Review for anti-Smad 5 antibody (C-term)

Application:WB

Sample:HeLa

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC