ARG55591
anti-Smad 6 antibody
anti-Smad 6 antibody for Flow cytometry,Western blot and Human
Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Smad 6 |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms |
| Tested Application | FACS, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Smad 6 |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide corresponding to aa. 358-386 (Center) of Human Smad 6. |
| Conjugation | Un-conjugated |
| Alternate Names | AOVD2; Smad6; MADH7; MADH6; Mothers against decapentaplegic homolog 6; Mothers against DPP homolog 6; hSMAD6; HsT17432; MAD homolog 6; SMAD 6; SMAD family member 6 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | HT-29 |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A and immunogen peptide. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O43541 Human Mothers against decapentaplegic homolog 6 |
|---|---|
| Gene Symbol | SMAD6 |
| Gene Full Name | SMAD family member 6 |
| Background | The protein encoded by this gene belongs to the SMAD family of proteins, which are related to Drosophila 'mothers against decapentaplegic' (Mad) and C. elegans Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions in the negative regulation of BMP and TGF-beta/activin-signalling. Multiple transcript variants have been found for this gene.[provided by RefSeq, Sep 2014] |
| Function | Acts as a mediator of TGF-beta and BMP antiflammatory activity. Suppresses IL1R-TLR signaling through its direct interaction with PEL1, preventing NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of proinflammatory genes. May block the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding. Binds to regulatory elements in target promoter regions. [UniProt] |
| Cellular Localization | Nucleus. |
| Research Area | Cell Biology and Cellular Response antibody; Developmental Biology antibody; Gene Regulation antibody; Signaling Transduction antibody |
| Calculated MW | 53 kDa |
| PTM | Phosphorylated by BMP type 1 receptor kinase and by PRKX. Monoubiquitinated at Lys-173 by the E2/E3 hybrid ubiquitin-protein ligase UBE2O, leading to reduced binding affinity for the activated BMP type I receptor ACVR1/ALK2, thereby enhancing BMP7 and regulating adipocyte differentiation (PubMed:23455153). Ubiquitinated by WWP1 (By similarity). Ubiquitinated by RNF165, promoting proteasomal degradation, leading to enhance the BMP-Smad signaling (By similarity). Arginine methylation by PRMT1, which is recruited by BMPR2, initiates BMP-Induced signaling and induces dissociation from the BMPR1B receptor at the cell surface leading to derepress downstream Smad1/Smad5 signaling. |
