ARG62624
anti-Smith Antigen antibody [Y12]
anti-Smith Antigen antibody [Y12] for Electron microscopy,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Rat,D. melanogaster ,Pig
Immune System antibody
Overview
| Product Description | Mouse Monoclonal antibody [Y12] recognizes Smith Antigen |
|---|---|
| Tested Reactivity | Hu, Rat, Dm, Pig |
| Tested Application | EM, ICC/IF, IHC-P, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | Y12 |
| Isotype | IgG3 |
| Target Name | Smith Antigen |
| Immunogen | The immunogen is generated using human Smith Antigen. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Note | ICC/IF: 1/250 WB: 0.5 - 1 µg/ml IHC-P: 1 - 2 µg/ml * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G purified |
| Buffer | 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 0.2% BSA |
| Concentration | 0.2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Research Area | Immune System antibody |
|---|
Clone References
U2AF1 mutations alter sequence specificity of pre-mRNA binding and splicing.
The conserved ubiquitin-like protein Hub1 plays a critical role in splicing in human cells.
Time-resolved quantitative proteomics implicates the core snRNP protein SmB together with SMN in neural trafficking.
Compromised paraspeckle formation as a pathogenic factor in FUSopathies.
Spliceosome integrity is defective in the motor neuron diseases ALS and SMA.
ICC/IF / Human
Differentially expressed, variant U1 snRNAs regulate gene expression in human cells.
IP / Human
Tudor staphylococcal nuclease (Tudor-SN) participates in small ribonucleoprotein (snRNP) assembly via interacting with symmetrically dimethylated Sm proteins.
WB /
Netting neutrophils induce endothelial damage, infiltrate tissues, and expose immunostimulatory molecules in systemic lupus erythematosus.
ICC/IF / Human
Combination of SMN trans-splicing and a neurotrophic factor increases the life span and body mass in a severe model of spinal muscular atrophy.
IP / Mouse
The inability of fully grown germinal vesicle stage oocyte cytoplasm to transcriptionally silence transferred transcribing nuclei.
ICC/IF / Mouse
Ribonucleoprotein assembly defects correlate with spinal muscular atrophy severity and preferentially affect a subset of spliceosomal snRNPs.
WB / Mouse
Mutations in splicing factor PRPF3, causing retinal degeneration, form detrimental aggregates in photoreceptor cells.
IHC / Human
ZPR1 is essential for survival and is required for localization of the survival motor neurons (SMN) protein to Cajal bodies.
IHC / Mouse
pICln inhibits snRNP biogenesis by binding core spliceosomal proteins.
IP / Dog
