ARG22613
anti-Sodium / Hydrogen Exchanger 1 antibody [4E9]
anti-Sodium / Hydrogen Exchanger 1 antibody [4E9] for Western blot and Mouse,Rat,Fish,Pig,Rabbit
Overview
| Product Description | Mouse Monoclonal antibody [4E9] recognizes Sodium / Hydrogen Exchanger 1 This antibody recognizes the Na+/H+ exchanger-1 (NHE1), a membrane protein involved in pH regulation and signal transduction.Mouse anti Porcine sodium/hydrogen exchanger 1 antibody, clone 4E9 recognizes NHE1 from the salamander Amphiuma tridactylum (McLean et al. 1999) and in the flounder Pseudopleuronectes americanus |
|---|---|
| Tested Reactivity | Ms, Rat, Fsh, Pig, Rb |
| Tested Application | WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 4E9 |
| Isotype | IgG1 |
| Target Name | Sodium / Hydrogen Exchanger 1 |
| Antigen Species | Pig |
| Immunogen | Maltose binding protein fusion protein containing the entire C-terminal, hydrophilic domain of porcine NHE1. |
| Conjugation | Un-conjugated |
| Alternate Names | Sodium/hydrogen exchanger 1; LIKNS; APNH; +; PPP1R143; Na; NHE-1; Solute carrier family 9 member 1; NHE1 |
Application Instructions
| Application Suggestion |
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| Application Note | WB: OBT1655 detects a band of approximately 100 kDa in human kidney lysates. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | PBS and 0.1% Sodium azide. |
| Preservative | 0.1% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SLC9A1 |
| Gene Full Name | solute carrier family 9, subfamily A (NHE1, cation proton antiporter 1), member 1 |
| Background | This gene encodes a Na+/H+ antiporter that is a member of the solute carrier family 9. The encoded protein is a plasma membrane transporter that is expressed in the kidney and intestine. This protein plays a central role in regulating pH homeostasis, cell migration and cell volume. This protein may also be involved in tumor growth. [provided by RefSeq, Sep 2011] |
| Function | Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. [UniProt] |
| Calculated MW | 91 kDa |
| PTM | O-glycosylated. Ubiquitinated, leading to its degradation by the proteasome. Ubiquitination is reduced by CHP1 (By similarity). |
