ARG63902
anti-Stathmin 1 antibody
anti-Stathmin 1 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Stathmin 1 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Rat |
| Tested Application | ELISA, IHC-P, WB |
| Specificity | This antibody is expected to cross-react with isoform 1 a (NP_005554.1; NP_981944.1; NP_981946.1) only. The three reported isoforms (NP_005554.1, NP_981944.1 and NP_981946.1) represent identical protein. |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Stathmin 1 |
| Antigen Species | Human |
| Immunogen | C-KNKESKDPADETEAD |
| Conjugation | Un-conjugated |
| Alternate Names | PP17; Prosolin; Stathmin; Protein Pr22; PR22; Lag; C1orf215; PP19; pp19; SMN; OP18; Leukemia-associated phosphoprotein p18; LAP18; pp17; Oncoprotein 18; Phosphoprotein p19; Op18; Metablastin |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009] |
| Research Area | Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 17 kDa |
| PTM | Many different phosphorylated forms are observed depending on specific combinations among the sites which can be phosphorylated. MAPK is responsible for the phosphorylation of stathmin in response to NGF. Phosphorylation at Ser-16 seems to be required for neuron polarization (By similarity). Phosphorylation at Ser-63 reduces tubulin binding 10-fold and suppresses the MT polymerization inhibition activity. |
