ARG52433
anti-Synaptobrevin antibody [SP10]
anti-Synaptobrevin antibody [SP10] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Bovine,Hamster,Pig,Rabbit
Neuroscience antibody; Signaling Transduction antibody
Overview
| Product Description | Mouse Monoclonal antibody [SP10] recognizes Synaptobrevin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov, Hm, Pig, Rb |
| Tested Application | IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SP10 |
| Isotype | IgM |
| Target Name | Synaptobrevin |
| Antigen Species | Human |
| Immunogen | Synaptic immunoprecipitate (crude) from human brain |
| Conjugation | Un-conjugated |
| Alternate Names | Vesicle-associated membrane protein 1; Synaptobrevin-1; SYB1; VAMP-1; SPAX1 |
Application Instructions
| Application Suggestion |
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| Application Note | The antibody is specific for the ~16kDa synaptobrevin protein in Western blots of Rat brain lysate. The antibody has also been demonstRated to work in immunohistochemistry on formalin fixed, vibRatome sections, but does not work on paraffin sections. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | IgM Purified |
| Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
| Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | VAMP1/2 |
| Gene Full Name | vesicle-associated membrane protein 1 (synaptobrevin 1) |
| Background | Synaptobrevin (aka VAMP) is an integral membrane protein of synaptic vesicles that plays a major role in the formation of larger SNARE complexes, along with SNAP-25 and syntaxin. Synaptobrevin has been shown to be essential for two fast synapse-specific membrane trafficking processes: fast exocytosis for neurotransmitter release and fast endocytosis that mediates rapid recycling of synaptic vesicles (Deak et al., 2004). Decreased levels of synaptobrevin in human hippocampus and cortex have been correlated with cognitive defects in Alzheimer’s disease (Sze et al., 2000). |
| Research Area | Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 13 kDa |
