ARG52434
anti-Synaptojanin 1 antibody [5H1]
anti-Synaptojanin 1 antibody [5H1] for Western blot,Immunohistochemistry and Rat
Neuroscience antibody
Overview
Product Description | Mouse Monoclonal antibody [5H1] recognizes Synaptojanin 1 |
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Tested Reactivity | Rat |
Tested Application | IHC, WB |
Host | Mouse |
Clonality | Monoclonal |
Clone | 5H1 |
Isotype | IgG1 |
Target Name | Synaptojanin 1 |
Antigen Species | Rat |
Immunogen | Recombinant protein from the C-terminal region of rat synaptojanin 1. |
Conjugation | Un-conjugated |
Alternate Names | PARK20; Synaptojanin-1; EC 3.1.3.36; Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 1; INPP5G |
Application Instructions
Application Suggestion |
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Application Note | Specific for the ~145k synaptojanin I protein in Western blots of Rat brain extracts. Immunolabeling blocked by preadsorption of antibody with the protein used to geneRate the antibody. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
Form | Liquid |
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Purification | Protein G purified |
Buffer | 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol |
Stabilizer | 0.1 mg/ml BSA, 50% Glycerol |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | SYNJ1 |
Gene Full Name | synaptojanin 1 |
Background | Synaptojanin is a phosphatidylinositol phosphatase involved in clathrinmediated endocytosis of synaptic vesicles. Synaptojanin 1 has two alternatively spliced isoforms; one that is ~ 145 kDA and is exclusively expressed in neurons, and the other ~ 170 kDa which is expressed in non-neuronal, peripheral tissues (Ramjaun AR & McPherson PS, 1996). The gene which encodes Synaptojanin 1, SYNJ1, has been mapped to chromosome 21 thus making it a candidate for involvement in Down’s syndrome (DS). It has recently been demonstrated that Ts65Dn mice (the most commonly used model of DS) have altered phosphatidylinositol-4,5- bisphosphate metabolism. This defect is rescued by restoring SYNJ1 to disomy in the Ts65Dn mice (Voronov SV et al., 2008). |
Research Area | Neuroscience antibody |
Calculated MW | 173 kDa |