ARG52434

anti-Synaptojanin 1 antibody [5H1]

anti-Synaptojanin 1 antibody [5H1] for Western blot,Immunohistochemistry and Rat

Neuroscience antibody

Overview

Product Description Mouse Monoclonal antibody [5H1] recognizes Synaptojanin 1
Tested Reactivity Rat
Tested Application IHC, WB
Host Mouse
Clonality Monoclonal
Clone 5H1
Isotype IgG1
Target Name Synaptojanin 1
Antigen Species Rat
Immunogen Recombinant protein from the C-terminal region of rat synaptojanin 1.
Conjugation Un-conjugated
Alternate Names PARK20; Synaptojanin-1; EC 3.1.3.36; Synaptic inositol 1,4,5-trisphosphate 5-phosphatase 1; INPP5G

Application Instructions

Application Suggestion
Tested Application Dilution
IHC1:100
WB1:1000
Application Note Specific for the ~145k synaptojanin I protein in Western blots of Rat brain extracts. Immunolabeling blocked by preadsorption of antibody with the protein used to geneRate the antibody.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Protein G purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 85238 Rat SYNJ1

Swiss-port # Q62910 Rat Synaptojanin-1

Gene Symbol SYNJ1
Gene Full Name synaptojanin 1
Background Synaptojanin is a phosphatidylinositol phosphatase involved in clathrinmediated endocytosis of synaptic vesicles. Synaptojanin 1 has two alternatively spliced isoforms; one that is ~ 145 kDA and is exclusively expressed in neurons, and the other ~ 170 kDa which is expressed in non-neuronal, peripheral tissues (Ramjaun AR & McPherson PS, 1996). The gene which encodes Synaptojanin 1, SYNJ1, has been mapped to chromosome 21 thus making it a candidate for involvement in Down’s syndrome (DS). It has recently been demonstrated that Ts65Dn mice (the most commonly used model of DS) have altered phosphatidylinositol-4,5- bisphosphate metabolism. This defect is rescued by restoring SYNJ1 to disomy in the Ts65Dn mice (Voronov SV et al., 2008).
Research Area Neuroscience antibody
Calculated MW 173 kDa