ARG22914

anti-Synaptophysin antibody [SP15]

anti-Synaptophysin antibody [SP15] for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Hamster,Human,Marmoset,Rat

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Overview

Product Description Mouse Monoclonal antibody [SP15] recognizes Synaptophysin
Mouse anti Human Synaptophysin antibody, clone SP15 recognizes human synaptophysin, also known as Major synaptic vesicle protein p38. Synaptophysin is a 313 amino acid synaptic vesicle associated protein of ~38kDa containing a single marvel domain. Synaptophysin expression is ubiquitous in synaptic vesicle membranes where it is the most abundant protein and is involved in the regulation of endocytosis (Kwon et al. 2011) Loss and atypical expression of synaptophysin is noted in conditions such as Huntindon's disease (Goto and Hirano 1990) and Alzheimer's disease (Masliah et al. 1989). Mutations in the synaptophysin gene have been identified and lead to a condition, Mental retardation, X-linked 96 (Tarpey et al. 2009).Mouse anti human synaptophysin antibody, clone SP15 has been used for the detection of synaptophysin by immunofluorescence, immunohistochemistry and western blotting on murine (Vitry et al. 2009), marmoset (Ramirez et al. 2001), rat (Barr et al. 2004), lapine (Roher et al. 2000) and human tissues (Beach et al. 1997).
Tested Reactivity Hu, Rat, Hm, Marmoset
Tested Application ELISA, IHC-P, WB
Host Mouse
Clonality Monoclonal
Clone SP15
Isotype IgM
Target Name Synaptophysin
Antigen Species Human
Immunogen Crude Human synaptic immunoprecipitate.
Conjugation Un-conjugated
Alternate Names Synaptophysin; MRXSYP; Major synaptic vesicle protein p38; MRX96

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
IHC-P1:25
WB1:500
Application Note IHC-P: Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified by gel filtration.
Buffer PBS and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 24804 Rat SYP

GeneID: 6855 Human SYP

Swiss-port # P07825 Rat Synaptophysin

Swiss-port # P08247 Human Synaptophysin

Gene Symbol SYP
Gene Full Name synaptophysin
Background This gene encodes an integral membrane protein of small synaptic vesicles in brain and endocrine cells. The protein also binds cholesterol and is thought to direct targeting of vesicle-associated membrane protein 2 (synaptobrevin) to intracellular compartments. Mutations in this gene are associated with X-linked mental retardation (XLMR). [provided by RefSeq, Aug 2011]
Function Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane. Involved in the regulation of short-term and long-term synaptic plasticity (By similarity). [UniProt]
Calculated MW 34 kDa
PTM Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.

Specific References

Cdc42 signaling regulated by dopamine D2 receptor correlatively links specific brain regions of hippocampus to cocaine addiction

IHC-Fr / Mouse

Juan Li et al.
Biochim Biophys Acta Mol Basis Dis.,  (2023)

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