ARG22798
anti-T Lymphocytes antibody [KEN-5]
anti-T Lymphocytes antibody [KEN-5] for ELISA,Flow cytometry,IHC-Frozen sections,Immunoprecipitation and Rabbit
Overview
| Product Description | Mouse Monoclonal antibody [KEN-5] recognizes T Lymphocytes This antibody recognizes Rabbit (lapine) T-cells. Clone KEN-5 recognizes >90% thymocytes and approximately 40-45% mesenteric lymph node cells and immunoprecipitates a 67 kDa protein from Rabbit thymocytes. In contrast to Humans and mice where only a very small population of B-cells express the CD5 antigen, the CD5 antigen in Rabbits is widely expressed by peripheral blood B cells. However, the KEN-5 antibody, while originally reported as recognising Rabbit CD5 (Kotani et al.1993), does not bind to Rabbit CD5 transfectants. Furthermore, clone KEN-5, unlike other known antibodies recognising Rabbit CD5 and anti Human cross reactive CD5 antibodies, shows very limited binding to Rabbit B-cells, which in adult Rabbits have been demonstrated to express CD5 (Raman & Knight 1992) More recently studies (Pospisil et al. 2009) indicate that clone KEN-5 may recognize a epitope that is dependant on glycosylation to maintain its structural configuration which may explain why this antibody does not recognize recombinant Rabbit CD5 produced in insect cells which contain different glycans from those found in mammalian cells. It has therefore been suggested that KEN-5 may distinguish between different isoforms of CD5 in lapine T and B cells (Pospisil et al. 2009). |
|---|---|
| Tested Reactivity | Rb |
| Tested Application | ELISA, FACS, IHC-Fr, IP |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | KEN-5 |
| Isotype | IgG1 |
| Target Name | T Lymphocytes |
| Antigen Species | Rabbit |
| Immunogen | Rabbit Thymocytes |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
