ARG41574
anti-TAF12 antibody
anti-TAF12 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes TAF12 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | TAF12 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to a sequence of Human TAF12. (QIADDFIESVVTAACQLARHRKSSTLEVKDVQLHLERQ) |
| Conjugation | Un-conjugated |
| Alternate Names | Transcription initiation factor TFIID subunit 12; TAFII20; TAF2J; TAFII20/TAFII15; Transcription initiation factor TFIID 20/15 kDa subunits; TAFII-20/TAFII-15 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 22 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q16514 Human Transcription initiation factor TFIID subunit 12 Swiss-port # Q8VE65 Mouse Transcription initiation factor TFIID subunit 12 |
|---|---|
| Gene Symbol | TAF12 |
| Gene Full Name | TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa |
| Background | Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2008] |
| Function | TAFs are components of the transcription factor IID (TFIID) complex, PCAF histone acetylase complex and TBP-free TAFII complex (TFTC). TAFs components-TIIFD are essential for mediating regulation of RNA polymerase transcription. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 18 kDa |
Images (5) Click the Picture to Zoom In
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ARG41574 anti-TAF12 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41574 anti-TAF12 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41574 anti-TAF12 antibody WB image
Western blot: 50 µg of samples under reducing conditions. Human placenta, U-937, K562 and THP-1 whole cell lysates stained with ARG41574 anti-TAF12 antibody at 0.5 µg/ml dilution, overnight at 4°C.
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ARG41574 anti-TAF12 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human mammary cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41574 anti-TAF12 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41574 anti-TAF12 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Mouse brain tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41574 anti-TAF12 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41574 anti-TAF12 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat brain tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41574 anti-TAF12 antibody at 1 µg/ml dilution, overnight at 4°C.
