ARG54636
anti-TBP / TATA Binding Protein antibody [1TBP18]
anti-TBP / TATA Binding Protein antibody [1TBP18] for Immunoprecipitation,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Gene Regulation antibody
Publication2
Overview
| Product Description | Mouse Monoclonal antibody [1TBP18] recognizes TBP / TATA Binding Protein |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Species Does Not React With | Dm, Xenopus, Yeast |
| Tested Application | IP, WB |
| Specificity | This antibody recognizes an epitope within amino acid residues 1-20 of human, mouse and rat TBP. It does not react with TBP from Drosophila, yeast, silk worm, or Xenopus. Other species not investigated. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1TBP18 |
| Isotype | IgG1 |
| Target Name | TBP / TATA Binding Protein |
| Antigen Species | Human |
| Immunogen | Recombinant human TBP |
| Epitope | 1-20 a.a. |
| Conjugation | Un-conjugated |
| Alternate Names | TATA-binding factor; SCA17; TATA sequence-binding protein; Transcription initiation factor TFIID TBP subunit; GTF2D1; TATA-box factor; TFIID; GTF2D; HDL4; TATA-box-binding protein |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | HeLa |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G affinity chromatography |
| Buffer | PBS (pH 7.4) |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TBP |
| Gene Full Name | TATA box binding protein |
| Background | The TATA-binding protein (TBP) plays acentral role in the assembly of mosteukaryotic transcription initiation complexes.TBP assembles with other proteins to formunique multimeric complexes for each of thethree different nuclear RNA polymerases.TBP has been cloned from a variety ofspecies and consists of two distinct domains.The C-terminal domain (~180 amino acids)is highly conserved among species,whereas the N-terminal domain variesconsiderably in length and sequence amongdifferent species. The conserved C-terminaldomain contains the DNA-binding region aswell as regions that interact with positive andnegative regulatory proteins. In human TBP,the non-conserved N-terminal domainincludes a stretch of glutamine residuesranging from 26 to 42. Several researchgroups have found an association betweenexpansion of this polyglutamine tract withrare forms of spinocerebellar ataxia andother neurodegenerative diseases including Huntington's disease. |
| Function | General transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA. [UniProt] |
| Research Area | Controls and Markers antibody; Gene Regulation antibody |
| Calculated MW | 38 kDa |
Customer's Feedback
Average
Review for anti-TBP / TATA Binding Protein antibody [1TBP18]
Application：WB
Sample：Rat heart
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
Review for anti-TBP / TATA Binding Protein antibody [1TBP18]
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
Review for anti-TBP / TATA Binding Protein antibody [1TBP18]
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
4-Acetylantrocamol LT3, a New Ubiquinone from Antrodia cinnamomea, Inhibits Hepatocellular Carcinoma HepG2 Cell Growth by Targeting YAP/TAZ, mTOR, and WNT/β-Catenin Signaling.
WB / Human
Rhodiola crenulata Suppresses High Glucose-Induced Matrix Metalloproteinase Expression and Inflammatory Responses by Inhibiting ROS-Related HMGB1-TLR4 Signaling in Endothelial Cells.
WB / Human
