ARG65529

anti-TCR alpha + beta antibody [R73]

anti-TCR alpha + beta antibody [R73] for Flow cytometry,Immunoprecipitation,Western blot,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,ICC/IF,Functional study and Primates,Rat

Developmental Biology antibody; Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [R73] recognizes TCR alpha + beta
Tested Reactivity Rat, NHuPrm
Tested Application FACS, FuncSt, ICC/IF, IHC-Fr, IHC-P, IP, WB
Specificity The clone R73 recognizes TCR alpha/beta, the dominant subtype of T cell receptor expressed in peripheral blood.
Host Mouse
Clonality Monoclonal
Clone R73
Isotype IgG1
Target Name TCR alpha + beta
Immunogen Rat T blasts and erythrocytes
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 12 µg/ml
FuncStAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
IHC-PAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note Functional studies: In vitro activation and in vivo depletion of TCR alpha/beta T cells.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from cell culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 24820 Rat TRA

Background The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression._x000D_
Research Area Developmental Biology antibody; Immune System antibody
Calculated MW TCR alpha: 30 kDa
TCR beta: 35 kDa