ARG63109

anti-TCR beta (Vbeta5.3 related) antibody [MEM-262]

anti-TCR beta (Vbeta5.3 related) antibody [MEM-262] for Flow cytometry,Functional study,Immunoprecipitation,Western blot and Human

Immune System antibody

Overview

Product Description Mouse Monoclonal antibody [MEM-262] recognizes TCR beta (Vbeta5.3 related)
Tested Reactivity Hu
Tested Application FACS, FuncSt, IP, WB
Specificity The clone MEM-262 recognizes beta chains of the TCR expressed by HPB-ALL cell line [carrying V(beta5.3)] and a small subset of peripheral blood T cells. This subset is larger than that recognized by other V(beta5.3)-specific antibodies.
Host Mouse
Clonality Monoclonal
Clone MEM-262
Isotype IgG2a
Target Name TCR beta (Vbeta5.3 related)
Antigen Species Human
Immunogen Human thymoma cell line HPB-ALL.
Conjugation Un-conjugated
Alternate Names TCRB; TRB@

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 - 4 µg/ml
FuncStAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note WB: Uniquely recognizes denatured TCR beta chains.
Functional studies: Activates T cells (V beta 5-related subset).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from hybridoma culture supernatant by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 6957 Human TRB

Gene Symbol TRB
Gene Full Name T cell receptor beta locus
Background T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor beta locus. The beta locus includes V (variable), J (joining), diversity (D), and C (constant) segments. During T cell development, the beta chain is synthesized by a recombination event at the DNA level joining a D segment with a J segment; a V segment is then joined to the D-J gene. The C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random additional of nucleotides by terminal deoxynucleotidyltransferase. Several V segments and one J segment of the beta locus are known to be incapable of encoding a protein and are considered pseudogenes. The beta locus also includes eight trypsinogen genes, three of which encode functional proteins and five of which are pseudogenes. Chromosomal abnormalities involving the T-cell receptor beta locus have been associated with T-cell lymphomas. [provided by RefSeq, Jul 2008]
Research Area Immune System antibody
Calculated MW 35 kDa