ARG23218

anti-TCR gamma + TCR delta antibody [GL3] (FITC)

anti-TCR gamma + TCR delta antibody [GL3] (FITC) for Flow cytometry and Mouse

Overview

Product Description FITC-conjugated Hamster Monoclonal antibody [GL3] recognizes TCR gamma + TCR delta
Hamster anti Mouse TCR Gamma/Delta antibody, clone GL3 reacts with mouse TCR gamma/delta expressing lymphocytes. Hamster anti Mouse TCR Gamma/Delta antibody, clone GL3 has been shown to have depleting activity in vivo (Van der Heyde et al. 1995 and  Skeenet al. 1993).
Tested Reactivity Ms
Tested Application FACS
Host Hamster
Clonality Monoclonal
Clone GL3
Isotype IgG
Target Name TCR gamma + TCR delta
Antigen Species Mouse
Immunogen C57BL/6J intra epithelial lymphocytes.
Conjugation FITC
Alternate Names TCR gamma: TCRG
TCR delta: TCRD; TCRDV1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:5 - 1:50
Application Note FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.09% Sodium azide and 0.5% BSA.
Preservative 0.09% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.1 mg/ml
Storage Instruction Aliquot and store in the dark at 2-8°C. Keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol TRG; TRD
Gene Full Name T cell receptor gamma locus; T cell receptor delta locus
Background TCR gamma: T cell receptors recognize foreign antigens which have been processed as small peptides and bound to major histocompatibility complex (MHC) molecules at the surface of antigen presenting cells (APC). Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci. This region represents the germline organization of the T cell receptor gamma locus. The gamma locus includes V (variable), J (joining), and C (constant) segments. During T cell development, the gamma chain is synthesized by a recombination event at the DNA level joining a V segment with a J segment; the C segment is later joined by splicing at the RNA level. Recombination of many different V segments with several J segments provides a wide range of antigen recognition. Additional diversity is attained by junctional diversity, resulting from the random addition of nucleotides by terminal deoxynucleotidyltransferase. Several V segments of the gamma locus are known to be incapable of encoding a protein and are considered pseudogenes. Somatic rearrangement of the gamma locus has been observed in T cells derived from patients with T cell leukemia and ataxia telangiectasia. [provided by RefSeq, Jul 2008]
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Calculated MW 20 kDa