ARG64788

anti-TDP43 antibody

anti-TDP43 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Gene Regulation antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody

Overview

Product Description Goat Polyclonal antibody recognizes TDP43
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Cow, Dog, Pig
Tested Application IHC-P, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name TDP43
Antigen Species Human
Immunogen C-HISNAEPKHNSNRQ
Conjugation Un-conjugated
Alternate Names TAR DNA-binding protein 43; TDP-43; ALS10

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P5 µg/ml
WB0.3 - 1 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 23435 Human TARDBP

Swiss-port # Q13148 Human TAR DNA-binding protein 43

Background HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq, Jul 2008]
Research Area Gene Regulation antibody; Microbiology and Infectious Disease antibody; Neuroscience antibody
Calculated MW 45 kDa
PTM Hyperphosphorylated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
Ubiquitinated in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.
Cleaved to generate C-terminal fragments in hippocampus, neocortex, and spinal cord from individuals affected with ALS and FTLDU.