ARG58890

anti-TFIIE antibody

anti-TFIIE antibody for ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes TFIIE
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TFIIE
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 230-439 of Human TFIIE (NP_005504.2).
Conjugation Un-conjugated
Alternate Names General transcription factor IIE subunit 1; General transcription factor IIE 56 kDa subunit; TF2E1; TFIIE-alpha; FE; TFIIE-A; Transcription initiation factor IIE subunit alpha

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:200 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa
Observed Size 60 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 2960 Human GTF2E1

GeneID: 74197 Mouse GTF2E1

Swiss-port # P29083 Human General transcription factor IIE subunit 1

Swiss-port # Q9D0D5 Mouse General transcription factor IIE subunit 1

Gene Symbol GTF2E1
Gene Full Name general transcription factor IIE, polypeptide 1, alpha 56kDa
Function Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 49 kDa