ARG22415

anti-TGN38 antibody

anti-TGN38 antibody for Electron microscopy,ICC/IF,IHC-Frozen sections,Western blot and Mouse,Rat

Overview

Product Description Sheep Polyclonal antibody recognizes TGN38
This antibody recognizes rat TGN38, a 357 amino acid single pass trans membrane glycoprotein found primarily in the Trans-golgi network, and acts as an excellent marker for this cellular organelle (Humphrey et al. 1993).TGN38 is likely to have a role in intracellular transport (McNamara et al. 2004) and plays a role in morphological maintenance (Girotti and Banting 1996). It is the homologue of human TGN46 and macaque TGN47 (Ponnambalam et al. 1996).
Tested Reactivity Ms, Rat
Tested Application EM, ICC/IF, IHC-Fr, WB
Host Sheep
Clonality Polyclonal
Isotype IgG
Target Name TGN38
Antigen Species Rat
Immunogen Recombinant fusion protein corresponding to extracellular domain of TGN38.
Conjugation Un-conjugated
Alternate Names Trans-Golgi network integral membrane protein TGN38; Tgoln1; Ttgn1; Tgn38

Application Instructions

Application Suggestion
Tested Application Dilution
EMAssay-dependent
ICC/IFAssay-dependent
IHC-Fr1:100 - 1:200
WB1:1000
Application Note IHC-Fr: Fixation with methanol or methanol/acetone recommended.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol Tgoln2
Gene Full Name trans-golgi network protein 2
Background resident integral membrane proteins of the trans-Golgi network (TGN), cycles constitutively between the TGN and the plasma membrane [RGD, Feb 2006]
Calculated MW 38 kDa