ARG64213

anti-TIA1 antibody

anti-TIA1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Immune System antibody

Overview

Product Description Goat Polyclonal antibody recognizes TIA1
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Dog
Tested Application ICC/IF, IHC-P, WB
Specificity This antibody is expected to recognise both reported isoforms (NP_071320.1 and NP_071505.1).
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name TIA1
Antigen Species Human
Immunogen C-PKSTYESNTKQ
Conjugation Un-conjugated
Alternate Names T-cell-restricted intracellular antigen-1; WDM; TIA-1; RNA-binding protein TIA-1; p40-TIA-1; Nucleolysin TIA-1 isoform p40

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF5 µg/ml
IHC-P3 - 5 µg/ml
WB0.1 - 0.3 µg/ml
Application Note WB: Recommend incubate at RT for 1h.
IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7072 Human TIA1

Swiss-port # P31483 Human Nucleolysin TIA-1 isoform p40

Background The product encoded by this gene is a member of a RNA-binding protein family and possesses nucleolytic activity against cytotoxic lymphocyte (CTL) target cells. It has been suggested that this protein may be involved in the induction of apoptosis as it preferentially recognizes poly(A) homopolymers and induces DNA fragmentation in CTL targets. The major granule-associated species is a 15-kDa protein that is thought to be derived from the carboxyl terminus of the 40-kDa product by proteolytic processing. Alternative splicing resulting in different isoforms of this gene product has been described in the literature. [provided by RefSeq, Jul 2008]
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Immune System antibody
Calculated MW 43 kDa