ARG62642

anti-TID1 antibody [RS11]

anti-TID1 antibody [RS11] for ICC/IF,Western blot and Human

Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [RS11] recognizes TID1
Tested Reactivity Hu
Tested Application ICC/IF, WB
Specificity Reacts with both short and long variants of TID1
Host Mouse
Clonality Monoclonal
Clone RS11
Isotype IgM, kappa
Target Name TID1
Antigen Species Human
Immunogen Recombinant full length protein (Human).
Conjugation Un-conjugated
Alternate Names hTid-1; TID1; DnaJ homolog subfamily A member 3, mitochondrial; DnaJ protein Tid-1; Hepatocellular carcinoma-associated antigen 57; Tumorous imaginal discs protein Tid56 homolog; hTID-1; HCA57

Application Instructions

Application Note IF: 1: 50-1: 500.
WB: 2 - 4ug / ml
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa cells, Jurka

Properties

Form Liquid
Buffer 10mM PBS (pH 7.4), 0.2% BSA and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Stabilizer 0.2% BSA
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 9093 Human DNAJA3

Swiss-port # Q96EY1 Human DnaJ homolog subfamily A member 3, mitochondrial

Gene Symbol DNAJA3
Gene Full Name DnaJ (Hsp40) homolog, subfamily A, member 3
Background TID-1 is a 52 kDa protein. The human TID-1 gene encodes two splice variants, TID-1L and TID-1S. TID-1L and TID-1S are localized to the mitochon-drial matrix where they regulate apoptotic signal transduction by affectingcytochrome c release and caspase-3 activation. Both TID-1L and TID-1S are cleaved at amino acid 66 upon entry into the mitochondria, indicating thatmature TID-1L and TID-1S represent cleavage products of cytoplasmic pre-proteins.
Function Modulates apoptotic signal transduction or effector structures within the mitochondrial matrix. Affect cytochrome C release from the mitochondria and caspase 3 activation, but not caspase 8 activation. Isoform 1 increases apoptosis triggered by both TNF and the DNA-damaging agent mytomycin C; in sharp contrast, isoform 2 suppresses apoptosis. Can modulate IFN-gamma-mediated transcriptional activity. Isoform 2 may play a role in neuromuscular junction development as an effector of the MUSK signaling pathway. [UniProt]
Cellular Localization Mitochondria
Research Area Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 52 kDa
PTM Tyrosine phosphorylated.