ARG42935

anti-TIMP2 antibody

anti-TIMP2 antibody for Western blot,ICC/IF and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes TIMP2
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TIMP2
Antigen Species Human
Immunogen Synthetic peptide within aa. 100-200 of Human TIMP2 (NP_003246.1).
Conjugation Un-conjugated
Alternate Names TIMP-2; Tissue inhibitor of metalloproteinases 2; CSC-21K; DDC8; Metalloproteinase inhibitor 2

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control A549
Observed Size ~ 26 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 29543 Rat TIMP2

GeneID: 7077 Human TIMP2

Swiss-port # P16035 Human Metalloproteinase inhibitor 2

Swiss-port # P30121 Rat Metalloproteinase inhibitor 2

Gene Symbol TIMP2
Gene Full Name TIMP metallopeptidase inhibitor 2
Background This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008]
Function Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. [UniProt]
Cellular Localization Secreted. [UniProt]
Calculated MW 24 kDa
PTM The activity of TIMP2 is dependent on the presence of disulfide bonds. [UniProt]