ARG58125

anti-TIMP3 antibody

anti-TIMP3 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human

Overview

Product Description Rabbit Polyclonal antibody recognizes TIMP3
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TIMP3
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa. 175-211 of Human TIMP3.
Conjugation Un-conjugated
Alternate Names HSMRK222; K222TA2; TIMP-3; Protein MIG-5; K222; SFD; Metalloproteinase inhibitor 3; Tissue inhibitor of metalloproteinases 3

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1 - 2 µg/ml
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM Citrate buffer (pH 6.0) for 10-20 min followed by cooling at RT for 20 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS (pH 7.4)
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7078 Human TIMP3

Swiss-port # P35625 Human Metalloproteinase inhibitor 3

Gene Symbol TIMP3
Gene Full Name TIMP metallopeptidase inhibitor 3
Background This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix (ECM). Expression of this gene is induced in response to mitogenic stimulation and this netrin domain-containing protein is localized to the ECM. Mutations in this gene have been associated with the autosomal dominant disorder Sorsby's fundus dystrophy. [provided by RefSeq, Jul 2008]
Function Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. May form part of a tissue-specific acute response to remodeling stimuli. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-9, MMP-13, MMP-14 and MMP-15. [UniProt]
Cellular Localization Cytoplasmic. [UniProt]
Calculated MW 24 kDa