ARG55409
anti-TIRAP antibody
anti-TIRAP antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cell Biology and Cellular Response antibody; Immune System antibody; Signaling Transduction antibody
Overview
Product Description | Rabbit Polyclonal antibody recognizes TIRAP |
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Tested Reactivity | Hu |
Predict Reactivity | Ms, Rat |
Tested Application | IHC-P, WB |
Specificity | At least three isoforms of TIRAP are know to exist; this antibody detects all three isoforms. |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | TIRAP |
Antigen Species | Mouse |
Immunogen | Synthetic peptide (15 aa) within the last 50 aa of Mouse TIRAP. |
Conjugation | Un-conjugated |
Alternate Names | Toll/interleukin-1 receptor domain-containing adapter protein; C130027E04Rik; TIR domain-containing adapter protein; Adaptor protein Wyatt; MyD88 adapter-like protein; Wyatt; Tlr4ap; AA407980; Mal |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
Positive Control | Human Heart Tissue Lysate | ||||||
Observed Size | ~ 35 kDa |
Properties
Form | Liquid |
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Purification | Affinity purification with immunogen. |
Buffer | PBS and 0.02% Sodium azide |
Preservative | 0.02% Sodium azide |
Concentration | 1 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links |
Swiss-port # P58753 Human Toll/interleukin-1 receptor domain-containing adapter protein |
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Gene Symbol | Tirap |
Gene Full Name | toll-interleukin 1 receptor (TIR) domain-containing adaptor protein |
Background | The innate immune system recognizes microbial pathogens through Toll-like receptors (TLRs), which identify pathogen-associated molecular patterns. Different TLRs recognize different pathogen-associated molecular patterns and all TLRs have a Toll-interleukin 1 receptor (TIR) domain, which is responsible for signal transduction. The protein encoded by this gene is a TIR adaptor protein involved in the TLR4 signaling pathway of the immune system. It activates NF-kappa-B, MAPK1, MAPK3 and JNK, which then results in cytokine secretion and the inflammatory response. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008] |
Function | Adapter involved in the TLR2 and TLR4 signaling pathways in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, MAPK1, MAPK3 and JNK, and resulting in cytokine secretion and the inflammatory response (By similarity). Positively regulates the production of TNF-alpha and interleukin-6 (By similarity). [UniProt] |
Research Area | Cell Biology and Cellular Response antibody; Immune System antibody; Signaling Transduction antibody |
Calculated MW | 24 kDa |
PTM | Phosphorylated by IRAK1 and IRAK4. Also phosphorylated by BTK. Polyubiquitinated. Polyubiquitination follows phosphorylation by BTK and leads to TIRAP degradation. |