ARG23284
anti-TLN1 / Talin 1 antibody [97H6]
anti-TLN1 / Talin 1 antibody [97H6] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rabbit
Overview
| Product Description | Mouse Monoclonal antibody [97H6] recognizes TLN1 / Talin 1 Mouse anti Human Talin-1 antibody, clone 97H6 recognizes an epitope within the region of amino acids 482-655 of humanTalin-1, a high molecular weight cytoskeletal protein, linking vinculin to the integrins, thereby linking the cytoskeleton to the extracellular matrix. It is specifically found in regions of cell-substratum contact and in lymphocytes at cell-cell contact points. Talin-1 plays a role in the assembly of actin filaments and in the migration of cells such as osteoclasts and fibroblasts. |
|---|---|
| Tested Reactivity | Hu, Ms, Rb |
| Tested Application | ICC/IF, IHC-P, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 97H6 |
| Isotype | IgG1 |
| Target Name | TLN1 / Talin 1 |
| Antigen Species | Human |
| Immunogen | His-tagged polypeptide containing aa. 489-911 of Mouse Talin 1. |
| Conjugation | Un-conjugated |
| Alternate Names | Talin-1; TLN; ILWEQ |
Application Instructions
| Application Suggestion |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | ICC/IF: Methanol treatment is required prior to antibody staining. IHC-P: Antigen Retrieval: Boil tissue section in Sodium citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TLN1 |
| Gene Full Name | talin 1 |
| Background | This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. [provided by RefSeq, Feb 2009] |
| Function | Probably involved in connections of major cytoskeletal structures to the plasma membrane. High molecular weight cytoskeletal protein concentrated at regions of cell-substratum contact and, in lymphocytes, at cell-cell contacts (By similarity). [UniProt] |
| Calculated MW | 270 kDa |
