ARG54946

anti-TLR9 antibody

anti-TLR9 antibody for ELISA,Immunohistochemistry,Western blot,ICC/IF and Human,Mouse

Immune System antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes TLR9
Tested Reactivity Hu, Ms
Tested Application ELISA, ICC/IF, IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TLR9
Antigen Species Human
Immunogen Synthetic peptide (16 aa) within aa. 960-1010 of Human TLR9.
Conjugation Un-conjugated
Alternate Names CD289; Toll-like receptor 9; CD antigen CD289

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IF10 μg/ml
IHC2 μg/ml
WB1 μg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse Spleen Tissue Lysate

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 54106 Human TLR9

GeneID: 81897 Mouse TLR9

Swiss-port # Q9EQU3 Mouse Toll-like receptor 9

Swiss-port # Q9NR96 Human Toll-like receptor 9

Gene Symbol TLR9
Gene Full Name toll-like receptor 9
Background TLR9 Antibody: Toll-like receptors (TLRs) are evolutionarily conserved pattern-recognition molecules resembling the toll proteins that mediate antimicrobial responses in Drosophila. These proteins recognize different microbial products during infection and serve as an important link between the innate and adaptive immune responses. TLR9 forms a subfamily along with TLR7 and TLR8 that recognize viral RNA and CpG DNA sequences and are localized in intracellular acidic compartments such as the phagolysosome. Unlike other TLRs which act through adaptor molecules such as TOLLIP, TIRAP, TRIF, and MyD88 to activate various kinases and transcription factors to respond to potential infection, TLR9 is strictly dependent on MyD88.
Function Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR9 is a nucleotide-sensing TLR which is activated by unmethylated cytidine-phosphate-guanosine (CpG) dinucleotides. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Controls lymphocyte response to Helicobacter infection. [UniProt]
Research Area Immune System antibody; Signaling Transduction antibody
Calculated MW 116 kDa
PTM Activated by proteolytic cleavage of the flexible loop between repeats LRR14 and LRR15 within the ectodomain. Cleavage requires UNC93B1. Proteolytically processed by first removing the majority of the ectodomain by either asparagine endopeptidase (AEP) or a cathepsin followed by a trimming event that is solely cathepsin mediated and required for optimal receptor signaling.