ARG53674

anti-TNFR2 antibody

anti-TNFR2 antibody for IHC-Formalin-fixed paraffin-embedded sections and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes TNFR2
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TNFR2
Antigen Species Mouse
Immunogen Synthetic peptide derived from C-terminus of mouse TNF-R2.
Conjugation Un-conjugated
Alternate Names TNFR1B; CD antigen CD120b; p75; CD120b; p75TNFR; TNF-R-II; TNFBR; TBPII; TNFR80; TBP-2; TNFR2; Tumor necrosis factor receptor 2; Tumor necrosis factor receptor type II; Etanercept; Tumor necrosis factor receptor superfamily member 1B; TNFR-II; TNF-R75; TNF-R2; TNF-RII; p80 TNF-alpha receptor

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 30 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Liver Carcinoma, Thymus

Properties

Form Liquid
Purification Immunogen affinity purified
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7133 Human TNFRSF1B

Swiss-port # P20333 Human Tumor necrosis factor receptor superfamily member 1B

Gene Symbol Tnfrsf1b
Gene Full Name tumor necrosis factor receptor superfamily, member 1b
Background The protein encoded by this gene is a member of the TNF-receptor superfamily. This protein and TNF-receptor 1 form a heterocomplex that mediates the recruitment of two anti-apoptotic proteins, c-IAP1 and c-IAP2, which possess E3 ubiquitin ligase activity. The function of IAPs in TNF-receptor signalling is unknown, however, c-IAP1 is thought to potentiate TNF-induced apoptosis by the ubiquitination and degradation of TNF-receptor-associated factor 2, which mediates anti-apoptotic signals. Knockout studies in mice also suggest a role of this protein in protecting neurons from apoptosis by stimulating antioxidative pathways. [provided by RefSeq, Jul 2008]
Function Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2 (By similarity). [UniProt]
Cellular Localization Cytoplasm
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Immune System antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 48 kDa
PTM Phosphorylated; mainly on serine residues and with a very low level on threonine residues.
A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing.