ARG23151
anti-TNFSF18 / GITRL antibody [YGL386]
anti-TNFSF18 / GITRL antibody [YGL386] for ELISA,Flow cytometry and Mouse
Overview
| Product Description | Rat Monoclonal antibody [YGL386] recognizes TNFSF18 / GITRL Rat anti Mouse GITRL antibody, clone YGL386 recognizes mouse glucocorticoid-induced tumour necrosis factor receptor ligand (GITRL), a 20 kD member of the tumour necrosis factor ligand superfamily, otherwise known as TNSF18. Mouse GITRL is predominantly expressed on immature and mature dendritic cells, macrophages and B cells. Expression of mouse GITRL can be transiently up regulated on these cells, following lipolysaccharide (LPS) stimulation, but protein expression is rapidly down modulated shortly after stimulation.Studies demonstrate that GITRL can neutralise the suppressive effects of CD4+CD25+ regulatory T cells and can also provide a co-stimulatory signal for both naïve and primed T cells, suggesting a key role for GITRL in the regulation of T cell-mediated immunity. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | ELISA, FACS |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | YGL386 |
| Isotype | IgG1 |
| Target Name | TNFSF18 / GITRL |
| Antigen Species | Mouse |
| Immunogen | Recombinant mouse GITRL. |
| Conjugation | Un-conjugated |
| Alternate Names | TL6; AITRL; Glucocorticoid-induced TNF-related ligand; Tumor necrosis factor ligand superfamily member 18; hGITRL; Activation-inducible TNF-related ligand; GITRL |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q7TS55 Mouse Tumor necrosis factor ligand superfamily member 18 |
|---|---|
| Gene Symbol | Tnfsf18 |
| Gene Full Name | tumor necrosis factor (ligand) superfamily, member 18 |
| Background | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for receptor TNFRSF18/AITR/GITR. It has been shown to modulate T lymphocyte survival in peripheral tissues. This cytokine is also found to be expressed in endothelial cells, and is thought to be important for interaction between T lymphocytes and endothelial cells. [provided by RefSeq, Jul 2008] |
| Function | Cytokine that binds to TNFRSF18/AITR/GITR. Regulates T-cell responses. Can function as costimulator and lower the threshold for T-cell activation and T-cell proliferation. Important for interactions between activated T-lymphocytes and endothelial cells. Mediates activation of NF-kappa-B. Triggers increased phosphorylation of STAT1 and up-regulates expression of VCAM1 and ICAM1. Promotes leukocyte adhesion to endothelial cells. Regulates migration of monocytes from the splenic reservoir to sites of inflammation (By similarity). [UniProt] |
| Calculated MW | 23 kDa |
