ARG57012

anti-TNNI1 antibody [36E7]

anti-TNNI1 antibody [36E7] for Western blot and Human

Overview

Product Description Mouse Monoclonal antibody [36E7] recognizes TNNI1
Tested Reactivity Hu
Tested Application WB
Host Mouse
Clonality Monoclonal
Clone 36E7
Isotype IgG2b, kappa
Target Name TNNI1
Antigen Species Human
Immunogen Recombinant fragment around aa. 1-187 of Human TNNI1.
Conjugation Un-conjugated
Alternate Names Troponin I, slow-twitch isoform; SSTNI; TNN1; Troponin I, slow skeletal muscle

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein A.
Buffer PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 10% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 7135 Human TNNI1

Swiss-port # P19237 Human Troponin I, slow skeletal muscle

Gene Symbol TNNI1
Gene Full Name troponin I type 1 (skeletal, slow)
Background Troponin proteins associate with tropomyosin and regulate the calcium sensitivity of the myofibril contractile apparatus of striated muscles. Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TnI-fast and TnI-slow genes are expressed in fast-twitch and slow-twitch skeletal muscle fibers, respectively, while the TnI-cardiac gene is expressed exclusively in cardiac muscle tissue. This gene encodes the Troponin-I-skeletal-slow-twitch protein. This gene is expressed in cardiac and skeletal muscle during early development but is restricted to slow-twitch skeletal muscle fibers in adults. The encoded protein prevents muscle contraction by inhibiting calcium-mediated conformational changes in actin-myosin complexes. [provided by RefSeq, Jul 2008]
Function Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. [UniProt]
Calculated MW 22 kDa