ARG66511
anti-TPO / Thyroid Peroxidase antibody
anti-TPO / Thyroid Peroxidase antibody for IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Mouse Monoclonal antibody recognizes TPO / Thyroid Peroxidase |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b, kappa |
| Target Name | TPO / Thyroid Peroxidase |
| Antigen Species | Human |
| Immunogen | Synthetic peptide derived from Human TPO / Thyroid Peroxidase. |
| Conjugation | Un-conjugated |
| Alternate Names | TPO; EC 1.11.1.8; TDH2A; Thyroid peroxidase; MSA; TPX |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Citric acid buffer (pH 6.0) was used. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol and 0.5% BSA |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TPO |
| Gene Full Name | thyroid peroxidase |
| Background | This gene encodes a membrane-bound glycoprotein. The encoded protein acts as an enzyme and plays a central role in thyroid gland function. The protein functions in the iodination of tyrosine residues in thyroglobulin and phenoxy-ester formation between pairs of iodinated tyrosines to generate the thyroid hormones, thyroxine and triiodothyronine. Mutations in this gene are associated with several disorders of thyroid hormonogenesis, including congenital hypothyroidism, congenital goiter, and thyroid hormone organification defect IIA. Multiple transcript variants encoding distinct isoforms have been identified for this gene, but the full-length nature of some variants has not been determined. [provided by RefSeq, May 2011] |
| Function | Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4). [UniProt] |
| Cellular Localization | Membrane; Single-pass type I membrane protein. Isoform 3: Cell surface. [UniProt] |
| Calculated MW | 103 kDa |
| PTM | Glycosylated. Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface. Cleaved in its N-terminal part. [UniProt] |
