ARG42649
anti-TPP1 antibody
anti-TPP1 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
Product Description | Rabbit Polyclonal antibody recognizes TPP1 |
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Tested Reactivity | Hu |
Tested Application | IHC-P, WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | TPP1 |
Antigen Species | Human |
Immunogen | Synthetic peptide corresponding to aa. 227-261 of Human TPP1. (CAQFLEQYFHDSDLAQFMRLFGGNFAHQASVARVV) |
Conjugation | Un-conjugated |
Alternate Names | EC 3.4.14.9; TPP-1; Tripeptidyl-peptidase I; GIG1; Lysosomal pepstatin-insensitive protease; SCAR7; Tripeptidyl aminopeptidase; Tripeptidyl-peptidase 1; CLN2; LPIC; Cell growth-inhibiting gene 1 protein; TPP-I |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
Positive Control | HeLa | ||||||
Observed Size | ~ 61 kDa |
Properties
Form | Liquid |
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Purification | Affinity purification with immunogen. |
Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Sodium azide and 5% BSA. |
Preservative | 0.05% Sodium azide |
Stabilizer | 5% BSA |
Concentration | 0.5 mg/ml |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links | |
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Gene Symbol | TPP1 |
Gene Full Name | tripeptidyl peptidase I |
Background | This gene encodes a member of the sedolisin family of serine proteases. The protease functions in the lysosome to cleave N-terminal tripeptides from substrates, and has weaker endopeptidase activity. It is synthesized as a catalytically-inactive enzyme which is activated and auto-proteolyzed upon acidification. Mutations in this gene result in late-infantile neuronal ceroid lipofuscinosis, which is associated with the failure to degrade specific neuropeptides and a subunit of ATP synthase in the lysosome. [provided by RefSeq, Jul 2008] |
Function | Lysosomal serine protease with tripeptidyl-peptidase I activity (PubMed:11054422, PubMed:19038966, PubMed:19038967). May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases (PubMed:11054422, PubMed:19038966, PubMed:19038967). Requires substrates with an unsubstituted N-terminus (PubMed:19038966). [UniProt] |
Cellular Localization | Lysosome. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV. [UniProt] |
Calculated MW | 61 kDa |
PTM | Activated by autocatalytic proteolytical processing upon acidification. N-glycosylation is required for processing and activity. [UniProt] |