ARG23275
anti-TREM1 antibody [L5-B8.2A12.3A12] (low endotoxin)
anti-TREM1 antibody [L5-B8.2A12.3A12] (low endotoxin) for IHC-Frozen sections,Flow cytometry,Functional study,ICC/IF,Western blot and Mouse
Overview
| Product Description | Azide free and low endotoxin Rat Monoclonal antibody [L5-B8.2A12.3A12] recognizes TREM1 Rat anti Mouse Trem-1 antibody, clone L5-B8. 2A12. 3A12 recognizes mouse triggering receptor expressed on myeloid cells 1 (TREM-1), a 30kD member of the immunoglobulin superfamily expressed on neutrophils and a subset of monocytes. TREM-1 is involved in the microbial inflammatory response. It acts in conjunction with the adaptor protein DAP12 to activate neutrophils and monocytes, leading to the secretion of pro-inflammatory mediators and enhancement of the inflammatory response. Microbial infection also induces the release of soluble form of TREM-1 from monocytes, which is reported to act as an inhibitor of TREM-1. TREM-1 mediates the septic shock pathway, and blocking TREM-1 has been reported to reduce inflammation and increase survival in certain models of bacterial infection. |
|---|---|
| Tested Reactivity | Ms |
| Tested Application | FACS, FuncSt, ICC/IF, IHC-Fr, WB |
| Host | Rat |
| Clonality | Monoclonal |
| Clone | L5-B8.2A12.3A12 |
| Isotype | IgG2a |
| Target Name | TREM1 |
| Antigen Species | Mouse |
| Immunogen | Mouse TREM-1 Fc fusion protein. |
| Conjugation | Un-conjugated |
| Alternate Names | TREM-1; CD antigen CD354; CD354; Triggering receptor expressed on monocytes 1; Triggering receptor expressed on myeloid cells 1 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-Fr: The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections. FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Purification Note | Low endotoxin |
| Buffer | PBS. |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9JKE2 Mouse Triggering receptor expressed on myeloid cells 1 |
|---|---|
| Gene Symbol | TREM1 |
| Gene Full Name | triggering receptor expressed on myeloid cells 1 |
| Background | This gene encodes a receptor belonging to the Ig superfamily that is expressed on myeloid cells. This protein amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011] |
| Function | Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock. [UniProt] |
| Calculated MW | 26 kDa |
| PTM | Glycosylated. [UniProt] |
