ARG23275

anti-TREM1 antibody [L5-B8.2A12.3A12] (low endotoxin)

anti-TREM1 antibody [L5-B8.2A12.3A12] (low endotoxin) for IHC-Frozen sections,Flow cytometry,Functional study,ICC/IF,Western blot and Mouse

Overview

Product Description Azide free and low endotoxin Rat Monoclonal antibody [L5-B8.2A12.3A12] recognizes TREM1
Rat anti Mouse Trem-1 antibody, clone L5-B8. 2A12. 3A12 recognizes mouse triggering receptor expressed on myeloid cells 1 (TREM-1), a 30kD member of the immunoglobulin superfamily expressed on neutrophils and a subset of monocytes. TREM-1 is involved in the microbial inflammatory response. It acts in conjunction with the adaptor protein DAP12 to activate neutrophils and monocytes, leading to the secretion of pro-inflammatory mediators and enhancement of the inflammatory response. Microbial infection also induces the release of soluble form of TREM-1 from monocytes, which is reported to act as an inhibitor of TREM-1. TREM-1 mediates the septic shock pathway, and blocking TREM-1 has been reported to reduce inflammation and increase survival in certain models of bacterial infection.
Tested Reactivity Ms
Tested Application FACS, FuncSt, ICC/IF, IHC-Fr, WB
Host Rat
Clonality Monoclonal
Clone L5-B8.2A12.3A12
Isotype IgG2a
Target Name TREM1
Antigen Species Mouse
Immunogen Mouse TREM-1 Fc fusion protein.
Conjugation Un-conjugated
Alternate Names TREM-1; CD antigen CD354; CD354; Triggering receptor expressed on monocytes 1; Triggering receptor expressed on myeloid cells 1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:25 - 1:100
FuncStAssay-dependent
ICC/IFAssay-dependent
IHC-FrAssay-dependent
WBAssay-dependent
Application Note IHC-Fr: The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. Arigo recommends the use of acetone fixation for frozen sections.
FACS: Use 10 µl of the suggested working dilution to label 10^6 cells in 100 µl.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Purification Note Low endotoxin
Buffer PBS.
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 58217 Mouse TREM1

Swiss-port # Q9JKE2 Mouse Triggering receptor expressed on myeloid cells 1

Gene Symbol TREM1
Gene Full Name triggering receptor expressed on myeloid cells 1
Background This gene encodes a receptor belonging to the Ig superfamily that is expressed on myeloid cells. This protein amplifies neutrophil and monocyte-mediated inflammatory responses triggered by bacterial and fungal infections by stimulating release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]
Function Stimulates neutrophil and monocyte-mediated inflammatory responses. Triggers release of pro-inflammatory chemokines and cytokines, as well as increased surface expression of cell activation markers. Amplifier of inflammatory responses that are triggered by bacterial and fungal infections and is a crucial mediator of septic shock. [UniProt]
Calculated MW 26 kDa
PTM Glycosylated. [UniProt]