ARG63116

anti-TRIM antibody [TRIM-04]

anti-TRIM antibody [TRIM-04] for Flow cytometry,Immunoprecipitation,Western blot and Human

Immune System antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [TRIM-04] recognizes TRIM
Tested Reactivity Hu
Species Does Not React With Ms
Tested Application FACS, IP, WB
Specificity The clone TRIM-04 reacts with T cell receptor-interacting molecule (TRIM), a 30 kDa adaptor protein expressed by T cells.
Host Mouse
Clonality Monoclonal
Clone TRIM-04
Isotype IgG2a
Target Name TRIM
Antigen Species Human
Immunogen Recombinant intracellular domain (aa 29-186) of human TRIM.
Conjugation Un-conjugated
Alternate Names TRIM; TCRIM; pp29/30; T-cell receptor-associated transmembrane adapter 1; T-cell receptor-interacting molecule; HSPC062

Application Instructions

Application Suggestion
Tested Application Dilution
FACSAssay-dependent
IPAssay-dependent
WBAssay-dependent
Application Note FACS: Staining technique: Intracellular staining.
WB: Under non-reducing condition.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 50852 Human TRAT1

Swiss-port # Q6PIZ9 Human T-cell receptor-associated transmembrane adapter 1

Gene Symbol TRAT1
Gene Full Name T cell receptor associated transmembrane adaptor 1
Background TRIM (T cell receptor-interacting molecule), also known as TRAT1 (T cell receptor associated transmembrane adaptor 1) is a 30 kDa protein expressed by T cells as a cystein-linked homodimer. It associates with TCR-CD3-zeta-chain complex and becomes phosphorylated by Src-family kinases. TRIM is potentially involved in negative regulation of TCR-mediated signaling, but its role remains unclear.
Function Stabilizes the TCR (T-cell antigen receptor)/CD3 complex at the surface of T-cells. [UniProt]
Research Area Immune System antibody; Signaling Transduction antibody
Calculated MW 21 kDa
PTM Phosphorylated on tyrosines by LCK or FYN upon TCR activation.

Clone References

Redundancy in antigen-presenting function of the HLA-DR and -DQ molecules in the multiple sclerosis-associated HLA-DR2 haplotype.

WB / Human

Sospedra M et al.
J Immunol.,  (2006)

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Developmentally regulated expression of the transmembrane adaptor protein trim in fetal and adult T cells.

Huynh T et al.
Scand J Immunol.,  (2001)

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The transmembrane adaptor protein TRIM regulates T cell receptor (TCR) expression and TCR-mediated signaling via an association with the TCR zeta chain.

Kirchgessner H et al.
J Exp Med.,  (2001)

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