ARG63260

anti-TRIM2 antibody

anti-TRIM2 antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat

Cell Biology and Cellular Response antibody; Gene Regulation antibody

Overview

Product Description Goat Polyclonal antibody recognizes TRIM2
Tested Reactivity Hu, Rat
Predict Reactivity Ms, Cow, Dog
Tested Application FACS, IHC-P, WB
Specificity This antibody is expected to recognise both reported isoforms (NP_056086.2; NP_001123539.1). Cross-reactivity with TRIM3 has been demonstrated.
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name TRIM2
Antigen Species Human
Immunogen DSGNHCFKVYRYLQ
Conjugation Un-conjugated
Alternate Names RNF86; EC 6.3.2.-; RING finger protein 86; Tripartite motif-containing protein 2; CMT2R; E3 ubiquitin-protein ligase TRIM2

Application Instructions

Application Suggestion
Tested Application Dilution
FACS10 µg/ml
IHC-P6 - 12 µg/ml
WB0.5 - 1 µg/ml
Application Note IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0).
WB: Recommend incubate at RT for 1h.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Buffer Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA
Preservative 0.02% Sodium azide
Stabilizer 0.5% BSA
Concentration 0.5 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 23321 Human TRIM2

GeneID: 361970 Rat TRIM2

Swiss-port # D3ZQG6 Rat Tripartite motif-containing protein 2

Swiss-port # Q9C040 Human Tripartite motif-containing protein 2

Background The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to cytoplasmic filaments. Its function has not been identified. [provided by RefSeq, Jul 2008]
Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody
Calculated MW 82 kDa
PTM RING-type zinc finger-dependent and UBE2D1-dependent autoubiquitination.