ARG58245

anti-TRIM23 antibody

anti-TRIM23 antibody for Western blot and Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes TRIM23
Tested Reactivity Ms, Rat
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TRIM23
Antigen Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-280 of Human TRIM23 (NP_001647.1).
Conjugation Un-conjugated
Alternate Names ARFD1; EC 6.3.2.-; RNF46; Tripartite motif-containing protein 23; E3 ubiquitin-protein ligase TRIM23; ARD1; ADP-ribosylation factor domain-containing protein 1; RING finger protein 46; GTP-binding protein ARD-1

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control PC12
Observed Size 64 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 81002 Rat TRIM23

GeneID: 81003 Mouse TRIM23

Swiss-port # P36407 Rat E3 ubiquitin-protein ligase TRIM23

Swiss-port # Q8BGX0 Mouse E3 ubiquitin-protein ligase TRIM23

Gene Symbol TRIM23
Gene Full Name tripartite motif containing 23
Background The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein is also a member of the ADP ribosylation factor family of guanine nucleotide-binding family of proteins. Its carboxy terminus contains an ADP-ribosylation factor domain and a guanine nucleotide binding site, while the amino terminus contains a GTPase activating protein domain which acts on the guanine nucleotide binding site. The protein localizes to lysosomes and the Golgi apparatus. It plays a role in the formation of intracellular transport vesicles, their movement from one compartment to another, and phopholipase D activation. Three alternatively spliced transcript variants for this gene have been described. [provided by RefSeq, Jul 2008]
Function Acts as an E3 ubiquitin-protein ligase. In the presence of the human cytomegalovirus (HCMV) protein UL144, participates in 'Lys-63'-linked auto-ubiquitination of TRAF6 resulting in the virally controlled activation of NF-kappa-B at early time of infection. The C-terminus can act as an allosteric activator of the cholera toxin catalytic subunit. [UniProt]
Cellular Localization Endomembrane system, Golgi apparatus membrane, Lysosome membrane. [UniProt]
Calculated MW 64 kDa