ARG40744

anti-TRIM25 antibody

anti-TRIM25 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF,Immunoprecipitation,Flow cytometry and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes TRIM25
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TRIM25
Antigen Species Human
Immunogen Synthetic peptide derived from Human TRIM25.
Conjugation Un-conjugated
Alternate Names EC 6.3.2.19; ZNF147; EFP; Ubiquitin/ISG15-conjugating enzyme TRIM25; Tripartite motif-containing protein 25; Z147; E3 ubiquitin/ISG15 ligase TRIM25; EC 6.3.2.n3; Zinc finger protein 147; Estrogen-responsive finger protein; RNF147; RING finger protein 147

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:60
ICC/IF1:100 - 1:500
IHC-P1:50 - 1:200
IP1:50
WB1:1000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 217069 Mouse TRIM25

GeneID: 7706 Human TRIM25

Swiss-port # Q14258 Human E3 ubiquitin/ISG15 ligase TRIM25

Swiss-port # Q61510 Mouse E3 ubiquitin/ISG15 ligase TRIM25

Gene Symbol TRIM25
Gene Full Name tripartite motif containing 25
Background The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq, Jul 2008]
Function Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. [UniProt]
Cellular Localization Cytoplasm. Note=Colocalized with DDX58 at cytoplasmic perinuclear bodies. [UniProt]
Calculated MW 71 kDa
PTM Auto-ISGylated. [UniProt]