ARG44758
anti-TRIM25 antibody
anti-TRIM25 antibody for Immunoprecipitation and Human
Overview
| Product Description | Mouse Monoclonal antibody recognizes TRIM25 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IP |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | TRIM25 |
| Antigen Species | Human |
| Conjugation | Un-conjugated |
| Alternate Names | EC 6.3.2.19; ZNF147; EFP; Ubiquitin/ISG15-conjugating enzyme TRIM25; Tripartite motif-containing protein 25; Z147; E3 ubiquitin/ISG15 ligase TRIM25; EC 6.3.2.n3; Zinc finger protein 147; Estrogen-responsive finger protein; RNF147; RING finger protein 147 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A purification |
| Buffer | PBS with 0.09% sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | TRIM25 |
| Gene Full Name | tripartite motif containing 25 |
| Background | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the cytoplasm. The presence of potential DNA-binding and dimerization-transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene. [provided by RefSeq, Jul 2008] |
| Function | Negative feedback regulator that controls excessive innate immune responses. Regulates both Toll-like receptor 4 (TLR4) and DDX58/RIG1-like helicases (RLH) pathways. May inhibit the LTR pathway by direct interaction with TRAF6 and attenuation of NF-kappa-B activation. May negatively regulate the RLH pathway downstream from MAVS and upstream of NF-kappa-B and IRF3 (By similarity). [UniProt] |
| Cellular Localization | Cytoplasm. Note=Colocalized with DDX58 at cytoplasmic perinuclear bodies. [UniProt] |
| Calculated MW | 71 kDa |
| PTM | Auto-ISGylated. [UniProt] |
