ARG44759
anti-TRIM5 antibody
anti-TRIM5 antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation and Human
Overview
| Product Description | Mouse Monoclonal antibody recognizes TRIM5 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P, IP |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Target Name | TRIM5 |
| Antigen Species | Human |
| Conjugation | Un-conjugated |
| Alternate Names | TRIM5; Tripartite Motif Containing 5; RNF88; TRIM5alpha; RING-Type E3 Ubiquitin Transferase TRIM5; Tripartite Motif-Containing Protein 5; Tripartite Motif Protein TRIM5; Tripartite Motif-Containing Protein 5 Alpha; Tripartite Motif Protein TRIM; Tripartite Motif-Containing 5; Ring Finger Protein 88; RING Finger Protein 88 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Protein A purification |
| Buffer | PBS with 0.09% sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9C035 Human Tripartite motif-containing protein 5 |
|---|---|
| Gene Symbol | TRIM5 |
| Gene Full Name | Tripartite Motif Containing 5 |
| Background | The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein forms homo-oligomers via the coilel-coil region and localizes to cytoplasmic bodies. It appears to function as a E3 ubiquitin-ligase and ubiqutinates itself to regulate its subcellular localization. It may play a role in retroviral restriction. Multiple alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
| Function | Functions as a ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. Promotes ISGylation of 14-3-3 sigma (SFN), an adapter protein implicated in the regulation of a large spectrum signaling pathway. Mediates estrogen action in various target organs. [UniProt] |
| Cellular Localization | Cytoplasm, Nucleus |
| Calculated MW | 56(alpha), 35(gamma), 33(delta), kDa |
| PTM | Acetylation, Phosphoprotein, Ubl conjugation |
