ARG40770

anti-TRIM63 / MuRF1 antibody

anti-TRIM63 / MuRF1 antibody for ICC/IF,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes TRIM63 / MuRF1
Tested Reactivity Hu, Ms, Rat
Predict Reactivity Pig
Tested Application ICC/IF, WB
Specificity This antibody detects 38 and 30 kDa proteins corresponding to the apparent molecular mass of MuRF1 isoforms on SDS-PAGE immunoblots of C2C12 cells, and detects a ~ 38 kDa band in mouse heart and muscle tissue.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name TRIM63 / MuRF1
Antigen Species Human
Immunogen KLH-conjugated synthetic peptide around the C-terminus of Human MuRF1.
Conjugation Un-conjugated
Alternate Names E3 ubiquitin-protein ligase TRIM63; Muscle-specific RING finger protein 1; EC 6.3.2.-; MURF2; MURF1; MuRF-1; Striated muscle RING zinc finger protein; SMRZ; RNF28; RING finger protein 28; Tripartite motif-containing protein 63; MuRF1; IRF; Iris RING finger protein

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:300
WB1:1000
Application Note WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 38 kDa

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.05% Sodium azide, 50% Glycerol and 1 mg/ml BSA.
Preservative 0.05% Sodium azide
Stabilizer 50% Glycerol and 1 mg/ml BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 140939 Rat TRIM63

GeneID: 84676 Human TRIM63

Swiss-port # Q91Z63 Rat E3 ubiquitin-protein ligase TRIM63

Swiss-port # Q969Q1 Human E3 ubiquitin-protein ligase TRIM63

Gene Symbol TRIM63
Gene Full Name tripartite motif containing 63, E3 ubiquitin protein ligase
Background This gene encodes a member of the RING zinc finger protein family found in striated muscle and iris. The product of this gene is an E3 ubiquitin ligase that localizes to the Z-line and M-line lattices of myofibrils. This protein plays an important role in the atrophy of skeletal and cardiac muscle and is required for the degradation of myosin heavy chain proteins, myosin light chain, myosin binding protein, and for muscle-type creatine kinase. [provided by RefSeq, Feb 2012]
Function E3 ubiquitin ligase. Mediates the ubiquitination and subsequent proteasomal degradation of CKM, GMEB1 and HIBADH. Regulates the proteasomal degradation of muscle proteins under amino acid starvation, where muscle protein is catabolized to provide other organs with amino acids. Inhibits de novo skeletal muscle protein synthesis under amino acid starvation. Regulates proteasomal degradation of cardiac troponin I/TNNI3 and probably of other sarcomeric-associated proteins. May play a role in striated muscle atrophy and hypertrophy by regulating an anti-hypertrophic PKC-mediated signaling pathway. May regulate the organization of myofibrils through TTN in muscle cells. [UniProt]
Cellular Localization Cytoplasm. Nucleus. Cytoplasm, myofibril, sarcomere, M line. Cytoplasm, myofibril, sarcomere, Z line. Note=Colocalizes with TNNI3 in myocytes (By similarity). Localizes to the M- and Z-lines in skeletal muscle. [UniProt]
Calculated MW 40 kDa